What''s Wrong With My Chromatography

2016-9-4 · Poorly Made Connections = Peak tailing/fronting If Dimension X is too short, a dead-volume, or mixing chamber, will occur. This can broaden or split peaks and/or cause tailing. It will typically affect all peaks, but especially early eluting isocratic peaks For information on making proper connections check out The LC Handbook, Pub. No. 5990-7595EN

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GC Troubleshooting Series Part Four: Tailing Peaks

2016-9-11 · Routine inlet maintenance is essential to preventing issues like tailing peaks. Possible Cause: Column and Inlet Maintenance. Sudden issues with tailing indicates column contamination, possibly caused by a recent dirty sample. Using sample prep, such as SPE, or filtration is a good idea to help avoid issues like peak tailing. And it can extend

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Variation of peak shape and peak tailing in ...

2013-3-7 · Analysis performed upon each peak yields itsretention factor and its USP tailing factor (defined as the ratio of two extensionsfrom the maximum point of a peak measured at 5% of the peak height). Variationof these input parameters allows the output to be varied between partition andadsorption and any combination between these extremes.

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Can anyone recommend a method for tailing ...

Peak tailing is mainly due to silanol interaction. So, if you are using basic compound add TEA 0.1%, it basically solves problem. Similarly while buying column select endcapped one.

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Normal Phase HPLC

This stationary phase was aimed to reduce peak tailing of basic compounds in SFC without the need for additives and to provide alternate selectivity. The introduction of this phase initiated a fairly consistent, progressive development of a number of phases by Princeton and other manufacturers (ES Industries, Phenomenex, Waters, etc.).

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Protein purification troubleshooting guide

2018-1-1 · Tailing peak Fronting peak. Protein recovery and activity * SEC = size exclusion chromatography, IEX = ion exchange chromatography, ... to remove trypsin-like serine proteases Protein adsorbed to filter during sample preparation • Use another type of filter with low protein

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What is Peak Tailing?

2014-8-8 · What is Peak Tailing? Request information. Aug 08 2014. An ideal chromatography peak is a nice sharp symmetrical shape, a Gaussian peak, on a flat baseline. A peak can deviate from this ideal in several different ways. It can become …

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[5] What do Chromatograms tell us? Peak Shape ...

A peak shape for the solvent peak is observed as shown in figure 1. After installing a new column, the solvent is tailing very strong while normally the peak looks like the trunk of a tree. The tail of the peak contains residual solvent molecules that are injected after the main injection is done. Very often the tail is caused by a dead volume.

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How can I prevent peak tailing in HPLC?

2011-1-1 · The first and foremost point to reduce tailing effect is the choice of class B silica based columns like Purospher STAR (offered by MerckMillipore) .This chemistry is polymeric resulting into ...

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Ghostly Peaks and other Haunting Aberrations in CE

2019-9-11 · problems like the ghost peak. In summary, the ghost peak is a persistent phenomenon that this community of CE Users has encountered extensively, made progress to control, but has yet to fully understand and eliminate. It was proposed that an inter-company collaboration be formed to once and for all determine the

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What is Endcapping?

What is Endcapping? > back to FAQ main page When we functionalize our silica it is impossible to react with all available silanol groups (free OH groups on the silica surface) so we "endcap" the residual ones with a capping agent such as trimethylsilylchloride to form …

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Peak Shape Functions: Gaussian

Translated into powder diffraction terms, the function for the intensity at any value of 2θ near the peak becomes: I(2θ) = I max exp [ − π (2θ − 2θ 0) 2 / β 2] where I max is the peak intensity, 2θ 0 is the 2θ position of the peak maximum, and the integral breadth, …

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Best Peak Shape Good Peak Shape in HPLC The ...

2015-9-4 · Increase in peak tailing Equilibrate with 50% ACN, then mobile phase . Slide 21 Consistent Retention of Procainamides on SB-Aq with Good Peak Shape min 0 10 15 5 min 0 10 15 5 Column: ZORBAX SB-Aq 4.6 x 150 mm, 5

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Perfect peak shape: Five solutions to peak ...

2020-11-12 · Cause 1: Firstly, tailing can occur when secondary interactions take place.As a result, not all molecules travel through the column at the same speed and this causing tailing at the peak. Possible Solutions: To remedy this, you could try to lower the pH of the liquids so that silanol ionization is suppressed (pH 3). Reducing the pH keeps the silanols in protonated form, where interaction with ...

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Peak tailing in SFC-MS (while good peak shape in ...

The mass spec is working fine. We got very good chromatogram and signal on another set of chiral isomers on the same SFC-MS system. Also, for the set of chiral isomers that have peak tailing issues, we tested on 2 different mass spec systems (a QTOF and a tripleQ), and they both showed the same poor peak shape with significant tailing.

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Peak Analyzer, Find Peaks Page

Use this to specify whether you want the Peak Analyzer to find the peaks automatically. When this check box is selected, the Add, Modify/Del, Clear All and Load buttons are disabled. Find. This button makes Origin find peaks using the current settings in the Find Peaks Settings and Peak Filtering groups. Add.

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2018-12-16 · Peak Tailing Caused by Stationary Phase Silanol Interactions with Amines A common cause of peak tailing in reversed phase HPLC is the secondary retention that occurs when an ion-exchange interaction takes place between a positively charged solute (amine) and an acidic silanol on the surface of silica stationary phase support particles ...

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HILIC peak tailing

 · I get a good retention time about 12 minutes but the peak shows heavy tailing, it basically looks like a kids slide, i have tried to change pH range from 2.5, 4.5 and 8.5 but the tailing keeps happening, tried using ammonium formate but got the same results, i also tried adding TEA to change the selectivity and perhaps help to dislocate the ...

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What is Peak Tailing?

A Tail of Two Peaks: Troubleshooting Poor Peak Shape

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HPLC Troubleshooting Cover

2006-7-4 · 6. Sources of Peak Tailing 7. Normal-Phase Chromatography 8. System Volume, Dead Volume, Dwell Volume 9. Transfer of Gradient Methods 10. Clogged System 11. Column Plate-Count 12. Column Backpressure 13. Peak Area Fluctuations 14. Ghost Peaks 15. Dependence of Retention Times on pH 16. Column Equilibration 17. Column Conditioning 18. Complex ...

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Tailing Comparisons

2021-9-29 · As the peak tailing increases, we become less and less happy with the results. If I consider the regulatory guidelines, peak tailing of up to ≈2.0 is allowed without raising too much concern. I have labelled peak tailing of 2.0 as undesirable (lower left …

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Peak Tailing

Brought the run time down to 1.2 mins. Peak tailing was around 1.3 which I was very happy about it.

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HPLC

6、(W h/2,peak withd at half height):,(1 HJ)。 7、(A,peak area):(1CHEJDC)。 8、(tailing peak):

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Protein purification troubleshooting guide

2021-3-3 · Tailing peak Fronting peak Tailing peak Fronting peak Home Peaks. Protein recovery is higher than expected Possible cause Remedy Proteins co-eluting with ... Flow (high sub) to remove trypsin-like serine proteases Protein adsorbed to filter during sample preparation • Use another type of filter with low protein binding (e.g., Whatman

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A Tail of Two Peaks: Troubleshooting Poor Peak Shape

2019-1-15 · Peak Tailing Injector or column is active • Reversible adsorption of active compounds (-OH, -NH, -SH) Flow problem • Dead volume, obstruction, poor installation, or severe column contamination Miscellaneous - overloading of PLOT columns, co-elution, polarity mismatch between phase, solute or solvent, and some compounds always tail

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Why is my chromatogram showing peak tailing ...

Peak Tailing. Peak tailing can occur due to numerous reasons. The problem can be identified according to the following scheme: Mass overload: when injecting less sample amount (mass) either the peak becomes more symmetrical or resolves into two separate peaks. Use a more dilute injection sample to correct the situation.

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Peak Tailing

 · I''m having an issue with peak tailing on an IC, that I can''t seem to resolve. It is a Dionex ICS-2000 with a KOH eluent generator cartridge, being used to analyze anions. The tailing is most noticeable on fluoride, nitrite, and ortho phosphate.

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Peak Tailing – Like an Iceberg, it ...

2013-3-20 · We found that peak tailing, like an iceberg, is much more harmful than commonly perceived. In GC-MS, peak tailing can originate at the GC column but the …

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HPLC Troubleshooting Guide

2017-2-14 · 1. Peak Shape 1.1. Peak tailinghas been the most common peak shape problem since the early days of RP-HPLC. Most peak tailing is due to interaction with acidic or ionized silanol groups on the surface of the silica particles within the column. The low-purity silica (often called "Type-A" or acidic silica) has a high content of

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MS Tip

Unacceptable solvent peak tailing and a poor reproducibility (see Table I) results. 0.2µl injection of 0.02% C10 in Heptane chromatographed on a 25QC3/BP1-0.5 column. Injector 200 degrees C and oven 120 degrees C. TABLE I - Reproducibility of 15 injections @0.3 µl. Centrally packed liner Liner packed to …

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HPLC: What to do in case of peaks being ...

2017-6-23 · Especially, the diameter of the capillary between the column and the detector has an enormous influence on the peak width - just like an old detector with a flow cell being too large (figure 2). Not only the flow cell but also the parameters of the detector can influence the peak width. Set the data acquisition rate (usually in Hertz) higher ...

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Tailing of Chromatographic Peaks in GC–MS ...

Fialkov et al. observed that the effect of ion source peak tailing is often underestimated, because most of the signal "is like an iceberg in the form of a long low amplitude tail" that may not be detectable. In this way, problems related to ion sources can reduce the chromatographic signals by more than one order of magnitude and with ...

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Application of mobile phase additives to ...

2019-9-15 · As shown in Fig. 2, the tailing factor at lower peak widths exhibits an exponential-like behavior making shallower gradients at the expense of longer run times and sensitivity a moot point.Upon closer inspection of Fig. 2, the observed deterioration of chromatographic performance over time suggests that the cause of tailing is being introduced in the form of a contaminant with adsorption ...

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Peak Shape Issues

These systems, like all chromatographic techniques, have a list of quite common problems seen by analysts. Missing peaks, tailing, carryover, fronting, inconsistent peak area, baseline noise, to name a few. If any of these terms seem familiar, don''t worry, …

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